HOPKINS WASHINGTON LIFE MEDICINE LAB
Research fields and concepts
Focus on Bio-Herbal Research
The HOPKINS WASHINGTON LIFE MEDICINE LAB has joined hands with experienced and accomplished cellular microbiologists and nutritionists to focus on research in the field of bio-herbs.
The concept of "Solving Health Issues with Nutrition
The laboratory has put forward the concept of "Solving Health Issues with Nutrition", transforming the achievements of bio-herbal research into high-quality nutritional foods, and improving sub-health and preventing diseases through nutritional supplements.
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Product Standards
and Supervision
Adhere to Strict Standards
Every box of the laboratory’s products adheres to strict standards of natural science, ensuring that product quality is strictly controlled from the source.
Full-Process Tracking and Supervision
From raw material procurement to the completion of product manufacturing, the laboratory conducts full-process tracking and supervision to ensure the efficient and high-quality transformation of premium formulas.
Research
Glutathione Supplementation of Parenteral Nutrition Prevents Oxidative Stressand Sustains Protein Synthesisin Guinea Pig Model
Abstract:
Peroxides in parenteral nutrition (PN) restrict methionine’s role as a cysteine precursor, causing cysteine deficiency. This deficiency is marked by low glutathione (key for peroxide detoxification) and limited growth in long-term PN recipients vs. the general population.
We hypothesized that glutathione supplementation in PN acts as a pro-cysteine, boosting glutathione levels, enhancing protein synthesis, and reducing PN-induced oxidative stress.
Using 1-month-old guinea pigs (7–8/group), we compared glutathione-enriched PN, non-enriched PN, and enteral nutrition (reference). PN included dextrose, amino acids (Primene), lipid emulsion (Intralipid), multivitamins, and electrolytes, infused for 5 days. We measured glutathione (GSH, GSSG, redox potential) and radioactive leucine incorporation (protein synthesis index) in blood, lungs, liver, and gastrocnemius muscle; data were analyzed via ANOVA (p<0.05 = significant).
Adding glutathione to PN prevented PN-induced oxidative stress in lungs/muscles and supported protein synthesis in liver/muscles. These results potentially support glutathione supplementation in PN and confirm glutathione as a bioavailable cysteine precursor.
Adding glutathione to parenteral nutrition prevents alveolar loss innewborn Guinea pig
Abstract:
Bronchopulmonary dysplasia (BPD), a major prematurity complication, is defined by alveolar hypoplasia. Premature infants have low endogenous glutathione (a key antioxidant) and face high oxidative stress (e.g., ascorbylperoxide from parenteral nutrition [PN]), suggesting oxidative stress triggers BPD.
Hypothesis: Adding glutathione (GSSG) to PN enhances ascorbylperoxide detoxification via glutathione peroxidase, thereby preventing excessive apoptosis and alveolar loss.
Methods: Ascorbylperoxide’s role as a glutathione peroxidase substrate was analyzed via Michaelis-Menten kinetics. Three-day-old guinea pig pups (6 groups) received IV infusions for 4 days: 1) Sham (no infusion); 2) PN(-L) (light-protected PN, low ascorbylperoxide); 3) PN(+L) (unprotected PN, high ascorbylperoxide); 4) 180 μM ascorbylperoxide; 5) PN(+L)+10 μM GSSG; 6) Ascorbylperoxide+10 μM GSSG. Lung samples were analyzed for histology and biochemistry; data via ANOVA (p<0.05 = significant).
Results: Glutathione peroxidase had a Km of 126±6 μM and Vmax of 38.4±2.5 nmol/min/U for ascorbylperoxide. GSSG in IV solutions prevented PN(+L)/ascorbylperoxide-induced effects: high GSSG redox potential, caspase-3 activation (apoptosis marker), and alveolar loss.
Conclusion: Correcting low glutathione in neonates on PN protects lungs from ascorbylperoxide toxicity. This is critical for premature infants (low glutathione) and offers potential for BPD prevention.
Glutathione in liver diseases and hepatotoxicity
Abstract:
Glutathione (GSH) is a major antioxidant as well as redox and cell signaling regulator. GSHguards cells against oxidative injury by reducing H,O, and scavenging reactive oxygen andnitrogen radicals. In addition, GSH-induced redox shift with or without ROS subjects somecellular proteins to varied forms of oxidation, altering the function of signal transductionand transcription factor molecules. Increasing evidence supports the important role ofROS and GSH in modulating multiple signaling pathways. TNF- and Fas signaling, NF-KB, |NK and mitochondrial apoptotic pathways are the focus of this review. The redox regulation either can switch on/off or regulate the threshold for some crucial events in thesepathways. Notably, mitochondrial GSH depletion induces increased mitochondrial ROsexposure which impairs bioenergetics and promotes mitochondrial permeability transitionpore opening which is critical for cell death, Depending on the extent of mitochondriadamage, NF-kB inhibition and jNK activation, hepatocytes may either undergo differentmodes of cell death (apoptosis or necrosis) or be sensitized to cell-death stimuli (i.eTNF-a). These processes have been implicated in the pathogenesis of many liver diseases
Damage During Acute Inflammation
Protective Role of Glutathione against Peroxynitrite-Mediated DNA
Abstract:
Inflammation is an immune response to protect against various types of infections. When unchecked, acuteinfammation can be life-threatening, as seen with the current coronavirus pandemic, Strong oxidants, such as peroxynitrite producedby immune cells, are major mediators of the infammation-associated pathogenesis. Cellular thiols play important roles in mitigatinginflammation-associated macromolecular damage including DNA. Herein, we have demonstrated a role of glutathione (GSH) andother thiols in neutralizing the effect of peroxynitrite -mediated DNA damage through stable GSH-DNA adduct formation. Ourobservation supports the use of thiol supplements as a potential therapeutic strategy against severe COVlD-19 cases and a Phase ll(NC'T04374461) open-abel clinical trial launched in early May 2020 by the Memorial Sloan Kettering Cancer Center.
Global Cooperation
CooperationOverview
The HOPKINS WASHINGTON LIFE MEDICINE LAB has established a cooperative relationship with Kidnow (U.S.) to jointly explore the field of health foods and health supplements.
TYMB® EGT15000 Complex Ergothioneine Tablets
Each bottle contains 60 Capsules (500mg). Each tablet contains 25mg of ergothioneine, made from natural mushroom extract. It has strong antioxidant properties and unique value in protecting cells from oxidative damage.
Hirudo Nipponica Freeze-Dried Powder Capsules
The product specification is 30 capsules (300mg) per unit. It supports a healthy blood system, maintains normal blood pressure, and is made with 100% natural ingredients.
Premium Crystal Tomato Whitening King
The product specification is 60 tablets per bottle. It contains six core natural ingredients including organic crystal tomatoes from South America and olive fruits from Germany. Visible whitening effects can be seen in 28 days, and each tablet contains 100mg of concentrated South American crystal white tomato extract.
NEWS
Hopkins Washington Life Medicine LAB has partnered with Kangjin Biotechnology to showcase their collaborative innovation in the global health industry at the China International Import Expo (CIIE).
At the 8th China International Import Expo (CIIE), the Hopkins Washington Life Medicine Laboratory sparked a wave of technological cooperation, signing in-depth strategic cooperation agreements with leading enterprises such as Hong Kong Kangjin Biotechnology and Australia AULINDA, embarking on a new journey of "Open Chain World · Cooperation Creates the Future". Adhering to the core philosophy of "Solving Health Problems with Nutrition", the laboratory leverages the CIIE platform to accelerate the transformation of cutting-edge scientific research achievements into practical applications, promoting the collaborative advancement of the global health industry.
